鲫鱼异倍体细胞系的建立及生物学特性

THE ESTABLISHMENT OF A HETEROPLOID LINE FROM CRUCIAN CARP AND ITS BIOLOGICAL CHARACTERISTICS

摘要:

在来源于鲫鱼囊胚细胞用含常量抗菌素的 TC 199培养基中加 20%小牛血清,于 27—28℃、pH 7.2条件下培养。经过 24个月继代培养 100次,建立一个命名为 CAB—80的鲫鱼异倍体细胞系。这个细胞系主要由上皮样细胞和少量成纤维样细胞组成。最适生长温度为22—28℃,最适 pH在 7.2—8.2之间,种群加倍时间为 21—45小时,接种 24小时后分裂指数达到29.‰,第60代细胞的染色体数目分布范围为68—178,因此,它是一个异倍体细胞系。CAB-80 细胞系对呼肠孤病毒的感染有一定的细胞病原效应,因此有可能作为鱼类病毒的一种检测材料。

Abstract:

This paper deals with the establiahment of a heteroploid cell line. designatedCAB--80, which originally comes from the culture of blastulae embryonic cells ofcrucian carp (Carassius auratus L.). Some biological characteristics of this cell lineare described briefly. The conditions used in primary cell culture and subculture areTC--199 medium containing a moderate amount of antibiotics and 20% calf serum andincubated at temperature 27--28℃,pH 7.2. A heteroploid cell line has ban established through 100 subculture passage over a period of 24 months.The cell line consists of epithelial like cells in a great measure and a few fibroblastlike cells.The optimum growth conditions of CAB--80 is at temperature 22--28℃ and pHvalue range at 7.2--8.2. The time for doubling CAB--80 cell population requires 21-45 hours. After incubation for 24 hours, the mitotic index of CAB--80 is 29.5‰. Thedistribution of chromosome numbers of 60th passage cells are ranged from 68 to 178,so it is heterploid cell line. The experiment shows that the CAB-80 cells have somewhat cytopathogenic effectto reovirus infection. It may be used as a bioassay indicator for fish virus.