第二届亚洲水产学术会议(1989.4.17～22)在日本东京召开

COMPARATIVE STUDY QN RESTRICTION ENDONUCLEASE DIGESTIUN OF mtDNAs FUR CARA55IUS AURATUS GIBELIC,CARAS5IU5 AURATUS CUYIERI AND CARA55IUS AURATUS AURATUS

摘要:

来自许多国家共约450名代表参加了这次盛会。出席人数以日本为最多,195名。我国47名(大陆10人)。菲律宾44名。来自亚洲国家和地区的还有印度、印尼、泰国、马来西业、新加坡、斯里兰卡、香港、科威特、孟加拉和尼泊尔的代表们;国际方面有加拿大、美国、澳大利亚、英国、法国、挪威,瑞典、希腊、巴西和苏丹等等15个国家的40名代表与会。

Abstract:

The mitochondrial DNAs (mtDNAs) of Carassius auratusbeZia,arassas auratus cuiera and Carassius auratus auratus Were digested by thirteenrestriction endonucleases, Via..Bam HI, Eco RI,13ra I,I,Hird III,Hpa TT,Msr I,Pst IAI, rna I, Xba I,I and rfaZ I. The double enzyme digestions wereconducted by using Bam HI, Eco RI, d III, Sma I, a I, SL I and Pst I. Thefragments Were obtained and their molecular sixes Were estimated. by aga.rase geIelectrophoresis. The size of mtDNAs for C. aecratusbeZin, C. auratus ceaiera and C.anratus auratusare 1599±90bp, 1600±130bp and 15540±140bp respectively.Taexperimental results demonstrated that the electrophoretic patterns of mtDNAsfor C. au-atusgibeaa, C. auratus cxiera and C. duratus aurdtus obtained by eithersingle or double enzyme digestion were significantly different, and the patternsof C. auraEusbebwv were obviously different among individuals by using Ha II.Besides, the restriction maps of mtDNAs from above species rere constructed byusing Bam HI, Pst I, Ecv RI and Xba I.