皱纹盘鲍淀粉酶和褐藻酸酶的研究

THE PROPERTIES OF AMYLASE AND ALGALASE IN HALIOTIS DISCUS HANNAI

摘要:

利用分光光度计比色法测定了２～３ｃｍ的皱纹盘鲍（ＨａｌｉｏｔｉｓｄｉｓｃｕｓｈａｎｎａｉＩｎｏ）的淀粉酶和褐藻酸酶的最适温度和最适ｐＨ以及１１种金属离子对其的影响。结果表明：最适温度分别为３０℃、３５℃，最适ｐＨ分别为６．２４、７．１９，在最适温度下的活化能分别为５．０３×１０４Ｊ／ｍｏｌ和１．３３×１０４Ｊ／ｍｏｌ；皱纹盘鲍的三种主要消化酶的活性依次为：褐藻酸酶＞蛋白酶＞淀粉酶。Ｃｕ２＋、Ｈｇ２＋、Ａｇ＋、Ｐｂ２＋对淀粉酶活性具有显著的抑制作用，其他离子则具有促进作用，其中又以Ｍｎ２＋、Ｂａ２＋、Ｃａ２＋三种金属离子尤为突出，其促进作用比对照组高一倍以上。而对褐藻酸酶，Ｚｎ２＋、Ａｇ＋、Ｈｇ２＋、Ｌｉ２＋、Ｂａ２＋具显著的抑制作用，其他离子则没有作用。

关键词:皱纹盘鲍 淀粉酶 褐藻酸酶 金属离子

Abstract:

The amylase and algalase of the Pacif ic abalone (Haliotis discus hannai) wer e measured during the length of 2 -3 cm by means of spect rophotome ter .The optimum tempe ratures f or amylase and algalase were 30 ℃ and 35 ℃, The opt imum pH values were 6 .24 and 7 .19 .Under the opt imum temper ature the act ive energy of two enzymes were 5 . 03 ×104 J/mol and 1 .33 ×104 J/mol r espect ively ;the act ivi ty of amylase and algalase were detected when eleven metal ions existed .The resul ts indicated that Cu2 + 、Hg2 、Ag 、Pb2 significantly inhibited the act ivi ty of amylase , and other metal ions activated the activity of amylase .Mn2 , Ba2 and Ca2 can act iva te the amylase two t imes of that in cont rol group .For the algalase , Zn2 , Ag , Hg2 , Li2 and Ba2 significant ly inhibited its activi ty , and the other metal ions had no effects on the algalase .

Key words:Haliotis discus hannai , Amylase , Algalase , Metal ions