锯缘青蟹脑内5-HT和NPY的免疫细胞化学定位(英文)

Immunocytochemical localization of serotonin and neuropeptide Y in the brain of Scylla serrata

摘要:

为探讨5-羟色胺和神经肽Y在锯缘青蟹脑内的存在与否和分布状况,应用免疫细胞化学技术,在光学显微镜下观察5-羟色胺和神经肽Y阳性细胞和神经髓质的形态和分布.结果表明: 在锯缘青蟹脑中共12个胞体群和11个神经髓质中,前脑和中脑中有4个细胞群和6个神经髓质检出5-HT免疫阳性反应; 中脑和后脑中有7个细胞群和3个神经髓质具有NPY免疫阳性反应.5-羟色胺和神经肽Y在锯缘青蟹脑内的特异性分布,为其参与神经生理活动提供了形态学证据.

Abstract:

To explore the locations of serotonin （5-HT） and neuropeptide Y （NPY） in the brain of Scylla serrata, immunocytochemical method of Strept Avidin-Biotin-Complex was applied to observe the immunoreaction to two antibodies. Among all the 12 somal clusters and 11 neuropils of the brain in S. serrata, 5-HT-like immunoreactivity occurs in 4 somal clusters and 6 neuropils in the protocerebrum and deutocerebrum; while NPY-like immunoreactivity is present in 7 somal dusters and 3 neuropils in the deutocerebrum and tritocerebrum. The specific distribution patterns of these two immunoreactive substances may thus provide morphological proofs for their different neurophysiological functions.