不同增殖方式来源的绿潮藻浒苔藻体生长及光合生理特性差异

The effects of different proliferative ways for the green tide alga Ulva prolifera on their growth and photophysiological performances

摘要:

为了探讨原位状态下不同增殖方式得到的绿潮藻浒苔生物体的差异，通过原位围隔实验，分别获取漂浮浒苔释放的孢子萌发形成的藻体（ST）和浒苔自身营养增殖得到的藻体（VT），通过比较这两种浒苔的生长、光合作用和荧光参数的不同，来估测其光合生理特性的差异。结果表明，ST的生长速率比VT显著高出61.27%，并且ST的最大光合作用速率（P_max）、光合效率（α）和光合活性（P/R）比VT分别显著高出25.33%、14.93%和134.69%，而呼吸作用速率和光补偿点比VT显著低了45.7%和52.2%，这表明ST与VT相比具有更大的生长优势。另外，VT的相对电子传递速率（rETR）显著高于ST，在高光下尤为显著，并且VT在高光下具有更高的非光化学猝灭（NPQ）能力，这说明浒苔通过自身营养增殖产生的藻体对高光的适应能力比其由孢子萌发形成的藻体更强。

关键词:浒苔;原位;光合作用;叶绿素荧光参数;生长

Abstract:

To compare the difference between Ulva prolifera cultured in different reproductive modes,we got the thalli grown from the spores(ST)and that from vegetative multiplication(VT)by using enclosure experiment in situ and then estimated the difference of photosynthetic performance between ST and VT by comparing the growth,photosynthesis and fluorescence parameter of VT and ST.Our results showed that the growth rate of ST was significantly higher than VT by 61.27% and the maximum photosynthetic rate Pmax,photosynthetic efficiency α and P/R of ST were significantly higher than those of VT by 25.33%,14.93%,134.69%,respectively.While the respiration rate and light compensation point for photosynthesis of ST were lower than those of VT by 45.7% and 52.2%,respectively.It showed that ST possessed better growth advantage compared with VT.Relative electron transport rates(rETR)and non-photochemical quenching(NPQ)of VT were significantly higher than those of ST,and had a higher non-photochemical quenching in the high light.These results implied that compared with the thalli grown from the spores,the thalli grown through vegetative multiplication had stronger high light adaptation ability.

Key words:Ulva prolifera;in situ;photosynthesis;chlorophyll florescence parameter;growth