黄鳍棘鲷家系亲缘关系鉴定

Establishment of parentage determination in yellowfin seabream (Acanthopagrus latus)

摘要:

为了建立黄鳍棘鲷微卫星亲子鉴定技术，利用荧光引物和自动测序技术检测了自主开发的12对微卫星分子标记在505尾黄鳍棘鲷个体中的遗传多态性，并构建了亲子鉴定技术。结果显示，该研究中筛选的12个微卫星标记共检测到119个等位基因，平均等位基因数（N_a）为9.91，平均观测杂合度（H_o）为0.651，平均期望杂合度（H_e）为0.661，平均多态性信息含量（PIC）为0.621，具有丰富的多态性。此外，运用Cervus 3.0 软件对已知系谱信息的112尾黄鳍棘鲷亲本和393尾子代个体进行模拟分析，结果显示，当双亲未知且置信度为95% 时，12 个标记的累积排除概率达99.58%；当微卫星标记数量为8 时，累积排除概率达到99.1%。因此确定AL49、AL37、AL01、AL20、AL14、AL18、AL15和AL51共8个多态性较高的微卫星标记为黄鳍棘鲷微卫星亲子鉴定的核心体系。在双亲性别未知的情况下，其双亲的累积排除率为99.1%。根据黄鳍棘鲷子代的实际基因分型数据，实际鉴定率为89.31%。该研究构建的微卫星标记组合能为黄鳍棘鲷不同家系混养后的亲子鉴定、种群选育和分子辅助家系管理提供科学的技术手段。

关键词:黄鳍棘鲷;微卫星;亲子鉴定;模拟分析

Abstract:

In this study, using twelve novel microsatellite markers, we established a paternity test technology for Acanthopagrus latus based on a mixed family of A. latus including 112 parent individuals and 393 offspring individuals. Genetic diversity data demonstrated that there were 119 alleles observed and the mean number of alleles (N_a), observed heterozygosity (H_o), expected heterozygosity (H_e) and polymorphism information content (PIC) were 9.91, 0.651, 0.661, 0.621, respectively. Paternity analysis showed that the combined probability of exclusion reached 99.58% when both parents were unknown (confidence level, 95%) by 12 loci. And, the combined probability of exclusion reached 99.1% when both parents were unknown (confidence level, 95%) by 8 loci. Consequently, the 8 high polymorphic microsatellite markers (AL49, AL37, AL01, AL20, AL14, AL18, AL15 and AL51) made up the system of paternity test for A. latus. The cumulative exclusion rate of their parents was 99.1% without parents' gender by 8 loci. The rate of assignment success for the real data set was only 89.31%. In conclusion, the combination of this novel 12 microsatellite markers in our study provided highly effective testing tools for the paternity identification, population breeding and molecular-assisted family management in A. Latus.

Key words:Acanthopagrus latus;microsatellites;parentage determination;simulation analysis