罗氏沼虾胚胎发育过程中的转录组

Transcriptome analysis during the development of Macrobrachium rosenbergii embryos

摘要:

目的 为了探索罗氏沼虾胚胎发育的分子调控机制。方法 本研究对罗氏沼虾胚胎的卵裂期Ⅰ、卵裂期Ⅱ、囊胚期、原肠胚期、前无节幼体、无节幼体、前蚤状幼体、蚤状幼体8个发育阶段进行转录组测序。结果 本研究共得到156.37 Gb clean data，预测得到22 964个unigenes。对罗氏沼虾8个不同发育阶段的胚胎样品的unigenes进行两两比较分析，获得各个时期之间的差异表达基因数量为587~8 620。对差异表达基因进行GO富集分析，上调表达基因主要富集在与细胞发育、生长、运动相关的GO功能类别，下调表达基因主要富集在与细胞的翻译调控、趋化、抗氧化、排毒活性相关的GO功能类别。KEEG富集分析发现，差异表达基因的pathway主要富集在与细胞的生长发育以及正常的生理生化过程相关的代谢途径。结论 本研究获得了罗氏沼虾胚胎的大量转录本信息，发现了与胚胎发育相关的差异表达基因及其主要富集功能和途径，为罗氏沼虾胚胎研究提供了有用的转录组信息资源。

Abstract:

Transcriptome sequencing was performed on eight developmental stages of Macrobrachium rosenbergii embryos, including cleavage stage Ⅰ, cleavage stage Ⅱ, blastocyst stage, gastrula stage, naupliusproprionalis, naupliusproprionalis, and flea like larva to explore the molecular regulatory mechanism of embryonic development of M. rosenbergii. A total of 156.37 Gb clean data was obtained, and 22 964 unigenes were predicted. A two-by-two comparative analysis of the embryonic samples from eight evelopmental stages of M. rosenbergii was performed, and the number of differentially expressed genes in eight embryonic samples of M. rosenbergii at different development stages were between 587 and 8 620. GO enrichment analysis of differentially expressed genes showed that up-regulated expressed genes were mainly enriched in GO functional categories related to cell development, growth, and motility. Meanwhile, the down-regulated expressed genes were mainly enriched in GO functional categories related to translational regulation, chemotaxis, antioxidant, and detoxification activities of cells. KEEG enrichment analysis found that differentially expressed genes pathways were mainly enriched in metabolic pathways related to cell growth and normal physiological and biochemical processes. This study obtained a large amount of transcriptome information on M. rosenbergii embryo and discovered differentially expressed genes related to embryonic development and their main enrichment functions and pathways, providing useful transcriptome resources for the embryo research of M. rosenbergii.